Step de l’acriflavine sur les levures
APPENDIX Within this appendix news and you may buffers needed for the fresh new actions put on preceding components of that it chapter are noted. Media
Slonimski, P
BMM. step 1.5 grams malt extract and you may 20 grams agar during the 1 L cornmeal pull. Cornmeal extract are obtained from 250 grams cornmeal incubated in 10 L liquids within 60°C right-away. After that timing this new supernatant is actually filtered through several levels from cheesecloth, additionally the cornmeal is actually discarded. CM medium. 0.15% MzP04,0.05% KCI, 0.05% MgS04,1% D- glucose, 0.37% NHEI, 0.2% Pepton, 0.2% fungus pull, step one m& ZnS04,l mg/L FeCb Buffers Denaturation bufler: step 1.5 M NaCI, 0.5 Meters NaOH Hybridization shield: 50% Formamide (stringent hybridization), 5 X SSPE, 0.5% salt dodecyl sulfate (SDS), 0.1 milligrams/mL salmon sperm DNA. (The newest stringency regarding hybridization ide). Continue reading